GlycoSens™ Aldehyde Dehydrogenase (ALDH) Activity Assay Kit, Micro-method

The GlycoSens™ Aldehyde Dehydrogenase (ALDH) Activity Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target aldehyde dehydrogenase activity in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Coenzyme I Series

ALDH

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

50 μL

The assay relies on the distinct spectroscopic optical characteristics of aldehyde dehydrogenase activity. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect ALDH activity in the sample.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Aldehyde Dehydrogenase

Dehydrogenase; ALDH; Oxidoreductase

Aldehyde dehydrogenase (ALDH) activity is an integral element required for robust cellular function and metabolism. It is responsible for driving the oxidative conversion of its substrate, typically utilizing NAD or FAD as cofactors. As a result, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Essential for monitoring aldehyde dehydrogenase activity level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.