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GlycoSens™ Bound Starch Synthase (GBSS) Assay Kit, Spectrophotometry

CAT#: GLJF-0926-HX637

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Specifications
Product Description
The GlycoSens™ Bound Starch Synthase (GBSS) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target bound starch synthase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Starch Series
Target
GBSS
Kit Components
Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer
Sample Types
Tissues, other biological samples
Sample Volume
20 μL - 100 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of bound starch synthase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect GBSS content in the sample.
Detection Time
Within 45 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Bound Starch Synthase
Alternate Names
Bound Starch Synthase; GBSS
Application Notes
Background
Found widely across biological samples, bound starch synthase (GBSS) is crucial for maintaining systemic homeostasis. It orchestrates the biosynthesis of complex biological molecules from simpler precursors. Consequently, assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Specifically engineered for evaluating bound starch synthase (GBSS) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
Key Features & Advantages
Superior Accuracy; Low Background Noise; Highly Stable Signal Output
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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