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The GlycoSens™ Bound Starch Synthase (GBSS) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target bound starch synthase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Carbohydrate Metabolism Series
Target
GBSS
Kit Components
Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of bound starch synthase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect GBSS content in the sample.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Bound Starch Synthase
Alternate Names
Bound Starch Synthase; GBSS
Application Notes
Background
As a pivotal biomolecule, bound starch synthase (GBSS) plays a central role in complex physiological networks. Its enzymatic function is indispensable for the efficient generation of cellular biomass and storage reserves. Consequently, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.
Application
Widely applicable for tracking bound starch synthase (GBSS) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.
Key Features & Advantages
Superior Accuracy; Low Background Noise; Highly Stable Signal Output
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.