GlycoSens™ Calcium/Magnesium-Dependent Adenosine Triphosphatase (Ca²⁺/Mg²⁺-ATPase) Assay Kit, Spectrophotometry

The GlycoSens™ Calcium/Magnesium-Dependent Adenosine Triphosphatase (Ca²⁺/Mg²⁺-ATPase) Assay Kit offers a highly reliable and sensitive spectrophotometric platform for quantifying Calcium/Magnesium-Dependent Adenosine Triphosphatase (Ca²⁺/Mg²⁺-ATPase) across varied biological matrices. Designed for rapid operation and high precision, this assay ensures reproducible quantification with low sample volume requirements.

Oxidative Phosphorylation Series

Ca²⁺/Mg²⁺-ATPase

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

Ca²⁺ Mg²⁺-ATPase breaks down ATP to produce ADP and inorganic phosphorus. The activity of ATPase is determined by measuring the amount of inorganic phosphorus.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Calcium/Magnesium-Dependent Adenosine Triphosphatase

Calcium/Magnesium-Dependent Adenosine Triphosphatase; Ca²⁺/Mg²⁺-ATPase; Calcium/Magnesium-Dependent Adenosine Triphosphatase Standard

Found widely across biological samples, calcium/magnesium-dependent adenosine triphosphatase (Ca²⁺/Mg²⁺-ATPase) is crucial for maintaining systemic homeostasis. By driving active ion exchange, it supports electrical excitability and secondary active transport processes. In addition, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Tailored for accurate quantification of calcium/magnesium-dependent adenosine triphosphatase (Ca²⁺ Mg²⁺-ATPase) across enzymology, membrane ion transport mechanics, and oxidative phosphorylation studies.

High Specificity; Rapid Reaction Protocol; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.