GlycoSens™ Chitinase Assay Kit, Colorimetry

The GlycoSens™ Chitinase Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of chitinase levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

Chitinase

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Cell lysates, tissue homogenates

10 μL - 50 μL

Quantification of chitinase is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 2 hours

585 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Chitinase

Chitinase

Known for its specific biological characteristics, chitinase provides deep insights into cellular dynamics. It effectively accelerates specific biochemical transformations, ensuring the rapid progression of metabolic pathways. Accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Specifically engineered for evaluating chitinase dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.