GlycoSens™ Chitinase Assay Kit, Spectrophotometry

The GlycoSens™ Chitinase Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target chitinase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Carbohydrate Metabolism Series

Chitinase

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

20 μL - 100 μL

The assay relies on the distinct spectroscopic optical characteristics of chitinase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect chitinase content in the sample.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Chitinase

Chitinase

Chitinase acts as an essential biochemical entity within diverse cellular pathways. It effectively accelerates specific biochemical transformations, ensuring the rapid progression of metabolic pathways. Moreover, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Specifically engineered for evaluating chitinase dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.