GlycoSens™ Chitinase Assay Kit, Micro-method

The GlycoSens™ Chitinase Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of chitinase levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Carbohydrate Metabolism Series

Chitinase

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

50 μL

Quantification of chitinase is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Chitinase

Chitinase

Chitinase acts as an essential biochemical entity within diverse cellular pathways. It selectively processes distinct biochemical substrates, maintaining the efficiency of cellular networks. Moreover, assessing this component offers a reliable parameter for agricultural research or biological studies.

Tailored for accurate quantification of chitinase across cell biology, organelle bioenergetics, and intracellular signaling cascades.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.