GlycoSens™ Creatine Kinase (CK) Assay Kit, Spectrophotometry

The GlycoSens™ Creatine Kinase (CK) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of creatine kinase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Coenzyme II Series

CK

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

Target creatine kinase (CK) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target CK.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Creatine Kinase

Creatine Kinase; CK; Creatine Phosphotransferase

Functioning at the core of various biological processes, creatine kinase (CK) is a key subject of biochemical analysis. By phosphorylating key molecular targets, it orchestrates complex intracellular communication networks. Notably, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Specifically engineered for evaluating creatine kinase (CK) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.