GlycoSens™ Creatine Kinase (CK) Assay Kit, Micro-method

The GlycoSens™ Creatine Kinase (CK) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target creatine kinase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Coenzyme II Series

CK

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of creatine kinase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect CK content in the sample.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Creatine Kinase

Creatine Kinase; CK; Creatine Phosphotransferase

Creatine kinase (CK) acts as an essential biochemical entity within diverse cellular pathways. This enzyme operates as a critical regulatory switch, modulating the activity of downstream signaling proteins. Detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Tailored for accurate quantification of creatine kinase (CK) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Enhanced Sensitivity; Stable Signal Output; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.