GlycoSens™ Creatine Kinase (CK) Assay Kit, Phosphomolybdate Method

The GlycoSens™ Creatine Kinase (CK) Assay Kit offers an advanced quantitative methodology for evaluating creatine kinase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Respiration and Energy Metabolism Series

CK

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

The system measures chromatic product formation derived from target creatine kinase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of CK levels.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Creatine Kinase

Creatine Kinase; CK; Creatine Phosphotransferase

In the context of biological research, creatine kinase (CK) has emerged as a molecule of high interest. It functions by transferring phosphate groups from high-energy donor molecules to specific target substrates. Notably, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Optimized for high-throughput profiling of creatine kinase (CK) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.