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The GlycoSens™ D-Mannose-6-phosphate (M6P) Assay Kit offers an advanced quantitative methodology for evaluating d-mannose-6-phosphate concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Other Series
Target
M6P
Kit Components
Sample Lysis Buffer; Substrate; Detection Reagent; Standard
Sample Types
Tissues, other biological samples
Sample Volume
5 μL - 20 μL
Detection Principle
The system measures chromatic product formation derived from target d-mannose-6-phosphate activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of M6P levels.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
D-Mannose-6-phosphate
Alternate Names
D-Mannose-6-phosphate; M6P
Application Notes
Background
D-Mannose-6-phosphate (M6P) acts as an essential biochemical entity within diverse cellular pathways. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. As a result, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.
Application
Essential for monitoring d-mannose-6-phosphate (M6P) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.
Key Features & Advantages
High Specificity; Micro-Volume Optimization; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.