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GlycoSens™ D-Mannose-6-phosphate Assay Kit, Microplate Method

CAT#: GLJF-0926-HX1502

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Specifications
Product Description
The GlycoSens™ D-Mannose-6-phosphate Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of d-mannose-6-phosphate levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Other Series
Target
M6P
Kit Components
Sample Lysis Buffer; Substrate; Detection Reagent; Standard
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
Quantification of d-mannose-6-phosphate is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
D-Mannose-6-phosphate
Alternate Names
D-Mannose-6-phosphate; M6P
Application Notes
Background
Found widely across biological samples, d-mannose-6-phosphate is crucial for maintaining systemic homeostasis. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Furthermore, assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Designed for comprehensive functional analysis of d-mannose-6-phosphate within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
Key Features & Advantages
Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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