GlycoSens™ Endogenous Hydrogen Sulfide (H₂S) Assay Kit

The GlycoSens™ Endogenous Hydrogen Sulfide (H₂S) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target endogenous hydrogen sulfide in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

H₂S

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of endogenous hydrogen sulfide. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect H₂S content in the sample.

Within 1 hour

665 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hydrogen Sulfide

Hydrogen Sulfide; H₂S; Endogenous Hydrogen Sulfide Standard; Endogenous Hydrogen Sulfide Reagent

As a pivotal biomolecule, endogenous hydrogen sulfide (H₂S) plays a central role in complex physiological networks. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Furthermore, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Tailored for accurate quantification of endogenous hydrogen sulfide (H₂S) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Stable Signal Output; High Sensitivity; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.