GlycoSens™ Fatty Acid Synthase (FAS) Assay Kit, Micro-method

The GlycoSens™ Fatty Acid Synthase (FAS) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target fatty acid synthase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Fatty Acid Metabolism Series

FAS

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of fatty acid synthase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect FAS content in the sample.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Fatty Acid Synthase

Fatty Acid Synthase; FAS

Found widely across biological samples, fatty acid synthase (FAS) is crucial for maintaining systemic homeostasis. It orchestrates the biosynthesis of complex biological molecules from simpler precursors. In addition, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Ideal for investigating fatty acid synthase (FAS) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.