GlycoSens™ Fatty Acid Synthase (FAS) Assay Kit, Spectrophotometry

The GlycoSens™ Fatty Acid Synthase (FAS) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of fatty acid synthase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Fatty Acid Metabolism Series

FAS

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

50 μL

Target fatty acid synthase (FAS) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target FAS.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Fatty Acid Synthase

Fatty Acid Synthase; FAS; Fatty Acid Synthetase

Fatty acid synthase (FAS) is recognized as a fundamental component in biological systems. By catalyzing the covalent linkage of its substrates, it supports intracellular synthesis requirements. Notably, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Essential for monitoring fatty acid synthase (FAS) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.