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GlycoSens™ Glucose Assay Kit, GOPOD Method

CAT#: GLJF-0926-HX1241

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Specifications
Product Description
The GlycoSens™ Glucose Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target glucose in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Basal Metabolic Series
Kit Components
Assay Buffer; Glucose Oxidase (GOD) Enzyme; Peroxidase (POD) Enzyme; Standard Solution
Sample Types
Serum, plasma, whole blood, cell culture supernatants, extracts
Sample Volume
10 μL - 50 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of glucose. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect glucose content in the sample.
Detection Time
Within 1 hour
Detection Wavelength
505 nm
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Application Notes
Background
The presence and activity of glucose are closely linked to overall organismal health. As a primary metabolic intermediate, it fuels diverse biosynthetic and catabolic reactions. Notably, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.
Application
Ideal for investigating glucose activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
Key Features & Advantages
High Specificity; Stable Reaction; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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