GlycoSens™ Nitrite Assay Kit, Spectrophotometry

The GlycoSens™ Nitrite Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of nitrite levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Nitrogen Metabolism Series

Nitrite

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

Quantification of nitrite is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Nitrite

Nitrite

Found widely across biological samples, nitrite is crucial for maintaining systemic homeostasis. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Consequently, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Specifically engineered for evaluating nitrite dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.