GlycoSens™ Threonine Assay Kit

The GlycoSens™ Threonine Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of threonine levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Amino Acid Metabolism Series

Threonine

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

10 μL - 50 μL

Quantification of threonine is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

High-performance liquid chromatograph, centrifuge, chromatographic column, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Threonine

Threonine

In the context of biological research, threonine has emerged as a molecule of high interest. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. Furthermore, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Essential for monitoring threonine level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Superior Accuracy; Low Background Noise; Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.