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GlycoSens™ Trehalose-6-phosphate Synthase (TPS) Assay Kit

CAT#: GLJF-0926-HX793

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Specifications
Product Description
The GlycoSens™ Trehalose-6-phosphate Synthase (TPS) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target trehalose-6-phosphate synthase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Carbohydrate Metabolism Series
Target
TPS
Kit Components
Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of trehalose-6-phosphate synthase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect TPS content in the sample.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Trehalose-6-phosphate Synthase
Alternate Names
Trehalose-6-phosphate Synthase; TPS
Application Notes
Background
Known for its specific biological characteristics, trehalose-6-phosphate synthase (TPS) provides deep insights into cellular dynamics. Its enzymatic function is indispensable for the efficient generation of cellular biomass and storage reserves. Notably, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.
Application
Ideal for investigating trehalose-6-phosphate synthase (TPS) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
Key Features & Advantages
Stable Signal Output; Stringent Quality Control; Enhanced Sensitivity
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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