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GlycoSens™ Hydroxyl Radical Scavenging Capacity Assay Kit

CAT#: GLJF-0926-HX1111

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Specifications
Product Description
The GlycoSens™ Hydroxyl Radical Scavenging Capacity Assay Kit offers an advanced quantitative methodology for evaluating hydroxyl radical scavenging capacity concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Oxidative Stress Series
Kit Components
Fenton Reaction Buffer; Fe2+ Reagent; Substrate Solution; Chromogenic Developer; Standard Solution
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
The system measures chromatic product formation derived from target hydroxyl radical scavenging capacity activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of hydroxyl radical scavenging capacity levels.
Detection Time
Within 1 hour
Detection Wavelength
510 nm / 536 nm
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Application Notes
Background
The presence and activity of hydroxyl radical scavenging capacity are closely linked to overall organismal health. This constituent influences multiple downstream effects, anchoring the stability of interrelated metabolic chains.
Application
Essential for monitoring hydroxyl radical scavenging capacity level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.
Key Features & Advantages
Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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