GlycoSens™ Soluble Acid Invertase (S-AI) Assay Kit, Micro-method

The GlycoSens™ Soluble Acid Invertase (S-AI) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of soluble acid invertase (S-AI) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Sucrose Series

S-AI

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

5 μL - 20 μL

Quantification of soluble acid invertase (S-AI) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Soluble Acid Invertase

Soluble Acid Invertase; S-AI

Research highlights soluble acid invertase (S-AI) as a significant participant in key metabolic and regulatory cascades. Its transport and utilization are critical for comprehensive physiological profiling and energy balance. As a result, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Essential for monitoring soluble acid invertase (S-AI) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.