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The GlycoSens™ Carnitine Palmitoyltransferase (CPT1) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of carnitine palmitoyltransferase (CPT1) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Quantification of carnitine palmitoyltransferase (CPT1) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Carnitine Palmitoyltransferase
Alternate Names
Transferase; CPT1; Carnitine Palmitoyltransferase
Application Notes
Background
Carnitine palmitoyltransferase (CPT1) acts as an essential biochemical entity within diverse cellular pathways. Its enzymatic activity is finely tuned to environmental cues, reflecting the overall metabolic capacity. Notably, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.
Application
Designed for comprehensive functional analysis of carnitine palmitoyltransferase (CPT1) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
Key Features & Advantages
High Specificity; Micro-Volume Optimization; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.