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The GlycoSens™ Soil Acid Protease (S-ACPT) Assay Kit offers an advanced quantitative methodology for evaluating soil acid protease concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Soil Series
Target
S-ACPT
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Soil, sediments, solid substrates
Sample Volume
10 μL - 50 μL
Detection Principle
The system measures chromatic product formation derived from target soil acid protease activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of S-ACPT levels.
Detection Time
Within 45 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Soil Acid Protease
Alternate Names
Soil Acid Protease; S-ACPT
Application Notes
Background
Soil acid protease (S-ACPT) is recognized as a fundamental component in biological systems. This parameter is vital for evaluating environmental changes and soil nutrient turnover. Moreover, assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Tailored for accurate quantification of soil acid protease (S-ACPT) across cell biology, organelle bioenergetics, and intracellular signaling cascades.
Key Features & Advantages
High Specificity; Micro-Volume Optimization; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.