GlycoSens™ Mitochondrial Respiratory Chain Complex III Assay Kit

The GlycoSens™ Mitochondrial Respiratory Chain Complex III Assay Kit offers a highly reliable and sensitive micro-volume spectrophotometric platform for quantifying mitochondrial respiratory chain complex III across varied biological matrices. Designed for rapid operation and high precision, this assay ensures reproducible quantification with low sample volume requirements.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex III

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

50 μL

Mitochondrial complex III, also known as CoQ-cytochrome C reductase, differs from oxidized cytochrome C. Reduced cytochrome C exhibits characteristic light absorption at 550 nm. Therefore, the rate of increase in light absorption at 550 nm can reflect the enzyme activity of mitochondrial complex III.

Within 1 hour

550 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex III

Complex III; Mitochondrial Respiratory Chain Complex III Standard; Mitochondrial Respiratory Chain Complex III Reagent; Coenzyme Q-Cytochrome C Reductase

In the context of biological research, mitochondrial respiratory chain complex III has emerged as a molecule of high interest. Operating at the nexus of energy metabolism, it significantly influences the oxidative phosphorylation capacity of the cell. Thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Ideal for investigating mitochondrial respiratory chain complex III activity and metabolic turnover during functional metabolomics, Q-cycle electron transfer analysis, and metabolic homeostasis research.

Stable Signal Output; Rapid Reaction Protocol; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.