GlycoSens™ Mitochondrial Respiratory Chain Complex III Assay Kit, Spectrophotometry

The GlycoSens™ Mitochondrial Respiratory Chain Complex III Assay Kit provides an advanced, quantitative spectrophotometric method for determining mitochondrial respiratory chain complex III in biological specimens. Featuring superior kinetic linearity and high throughput compatibility, it facilitates accurate biochemical profiling.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex III

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

50 μL

Mitochondrial complex III, also known as CoQ-cytochrome C reductase, differs from oxidized cytochrome C. Reduced cytochrome C exhibits characteristic light absorption at 550 nm, therefore, the rate of increase in light absorption at 550 nm can reflect the enzyme activity of mitochondrial complex III.

Within 1 hour

550 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex III

Complex III; Mitochondrial Respiratory Chain Complex III Standard; Mitochondrial Respiratory Chain Complex III Reagent; CoQ cytochrome C reductase

Mitochondrial respiratory chain complex III is recognized as a fundamental component in biological systems. It functions as an essential multi-subunit structure within the mitochondrial electron transport chain. Evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Ideal for investigating mitochondrial respiratory chain complex III activity and metabolic turnover during functional metabolomics, organelle fraction analysis, and metabolic homeostasis research.

Rapid Reaction Dynamics; High Specificity; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.