GlycoSens™ Phosphodiesterase (PDE) Assay Kit

The GlycoSens™ Phosphodiesterase (PDE) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target phosphodiesterase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Fatty Acid Series

PDE

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of phosphodiesterase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect PDE content in the sample.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Phosphodiesterase

Phosphodiesterase; PDE

Research highlights phosphodiesterase (PDE) as a significant participant in key metabolic and regulatory cascades. By selectively hydrolyzing complex molecules, it regulates the availability of active biological monomers. Evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Widely applicable for tracking phosphodiesterase (PDE) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.