GlycoSens™ Proline (Pro) Assay Kit

The GlycoSens™ Proline (Pro) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of proline (Pro) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

Pro

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, cellular lysates, other biological samples

10 μL - 50 μL

Quantification of proline (Pro) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Proline

Proline; Pro

In the context of biological research, proline (Pro) has emerged as a molecule of high interest. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Optimized for high-throughput profiling of proline (Pro) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.