GlycoSens™ Proline (PRO) Assay Kit, Micro-method

The GlycoSens™ Proline (PRO) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target proline in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

PRO

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Cell lysates, biological fluids

5 μL - 20 μL

The assay relies on the distinct spectroscopic optical characteristics of proline. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect PRO content in the sample.

30-60 minutes

520 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Proline

Proline; PRO

Proline (PRO) is an integral element required for robust cellular function and metabolism. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. In addition, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Ideal for investigating proline (PRO) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.