GlycoSens™ Proline (PRO) Assay Kit, Spectrophotometry

The GlycoSens™ Proline (PRO) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of proline. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Oxidation and Antioxidant Series

PRO

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Serum, plasma, tissues, cell culture supernatants

10 μL - 50 μL

Target proline (PRO) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target PRO.

30-60 minutes

520 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Proline

Proline; PRO

In the context of biological research, proline (PRO) has emerged as a molecule of high interest. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Consequently, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Designed for comprehensive functional analysis of proline (PRO) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.