GlycoSens™ Soluble Acid Invertase (S-AI) Assay Kit, Spectrophotometry

The GlycoSens™ Soluble Acid Invertase (S-AI) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target soluble acid invertase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Sucrose Series

S-AI

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

The assay relies on the distinct spectroscopic optical characteristics of soluble acid invertase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect S-AI content in the sample.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Soluble Acid Invertase

Soluble Acid Invertase; S-AI

Functioning at the core of various biological processes, soluble acid invertase (S-AI) is a key subject of biochemical analysis. As a key acidic metabolite, it modulates enzymatic activities and influences intra-cellular signaling. In addition, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Specifically engineered for evaluating soluble acid invertase (S-AI) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.