GlycoSens™ Soluble Acid Invertase (S-AI) Assay Kit

The GlycoSens™ Soluble Acid Invertase (S-AI) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of soluble acid invertase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Carbohydrate Metabolism Series

S-AI

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

20 μL - 100 μL

Target soluble acid invertase (S-AI) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target S-AI.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Soluble Acid Invertase

Soluble Acid Invertase/ Invertase; S-AI

As a pivotal biomolecule, soluble acid invertase (S-AI) plays a central role in complex physiological networks. It functions as a vital organic intermediate, connecting multiple energy-yielding metabolic cycles. In addition, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Essential for monitoring soluble acid invertase (S-AI) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.