To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
The GlycoSens™ Succinate Dehydrogenase (SDH) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of succinate dehydrogenase (SDH) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Quantification of succinate dehydrogenase (SDH) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
As a pivotal biomolecule, succinate dehydrogenase (SDH) plays a central role in complex physiological networks. By mediating the transfer of electrons, it strictly regulates intermediate metabolic fluxes. Furthermore, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.
Application
Tailored for accurate quantification of succinate dehydrogenase (SDH) across cell biology, organelle bioenergetics, and intracellular signaling cascades.
Key Features & Advantages
High Specificity; Excellent Reproducibility; Minimized Interference
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.