GlycoSens™ Succinate Dehydrogenase (SDH) Assay Kit, Micro-method

The GlycoSens™ Succinate Dehydrogenase (SDH) Assay Kit offers an advanced quantitative methodology for evaluating succinate dehydrogenase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Tricarboxylic Acid Cycle Series

SDH

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

The system measures chromatic product formation derived from target succinate dehydrogenase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of SDH levels.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Succinate Dehydrogenase

Succinate Dehydrogenase; SDH; Succinate Oxidoreductase

Known for its specific biological characteristics, succinate dehydrogenase (SDH) provides deep insights into cellular dynamics. This enzyme facilitates essential dehydrogenation steps required for cellular respiration and energy production. Furthermore, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Essential for monitoring succinate dehydrogenase (SDH) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.