GlycoSens™ Total Antioxidant Capacity (DPPH Method) Assay Kit, Spectrophotometry

The GlycoSens™ Total Antioxidant Capacity Assay Kit offers an advanced quantitative methodology for evaluating total antioxidant capacity concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Oxidation and Antioxidant Series

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

This assay relies on the unique spectroscopic optical signature of total antioxidant capacity. After reacting with specialized chromogenic reagents, the measured optical density change directly reflects the total antioxidant capacity of the sample.

Within 1 hour

515 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

As a pivotal biomolecule, total antioxidant capacity plays a central role in complex physiological networks. This collective metric is highly reflective of broad metabolic shifts and systemic physiological adaptations. In addition, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Designed for comprehensive functional analysis of total antioxidant capacity within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

High Specificity; Broad Sample Compatibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.