GlycoSens™ Trehalase Assay Kit

The GlycoSens™ Trehalase Assay Kit utilizes a highly stable detection system tailored for accurate profiling of trehalase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Carbohydrate Metabolism Series

Trehalase

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

5 μL - 20 μL

Target trehalase in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target trehalase.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Trehalase

Trehalase

Found widely across biological samples, trehalase is crucial for maintaining systemic homeostasis. Its enzymatic activity is finely tuned to environmental cues, reflecting the overall metabolic capacity. In addition, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Tailored for accurate quantification of trehalase across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.