GlycoSens™ Trehalase Assay Kit, Colorimetry

The GlycoSens™ Trehalase Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target trehalase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

Trehalase

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of trehalase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect trehalase content in the sample.

Within 1 hour

505 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Trehalase

Trehalase; Trehalase Standard; Trehalase Reagent

In the context of biological research, trehalase has emerged as a molecule of high interest. This catalytic protein strictly regulates substrate turnover to meet the dynamic physiological needs of the cell. Consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Essential for monitoring trehalase level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Stable Signal Output; Rapid Reaction Protocol; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.