GlycoSens™ Trehalose-6-phosphate Synthase (TPS) Assay Kit, Micro-method

The GlycoSens™ Trehalose-6-phosphate Synthase (TPS) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of trehalose-6-phosphate synthase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Trehalose Series

TPS

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

10 μL - 50 μL

Target trehalose-6-phosphate synthase (TPS) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target TPS.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Trehalose-6-phosphate Synthase

Trehalose-6-phosphate Synthase; TPS

Trehalose-6-phosphate synthase (TPS) is an integral element required for robust cellular function and metabolism. It acts as a vital synthetic engine, facilitating the formation of crucial biochemical intermediates. Moreover, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Essential for monitoring trehalose-6-phosphate synthase (TPS) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.