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Quantitative Human CMA1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX199

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human CMA1 (glycosylated) sandwich ELISA kit is designed to detect human mast cell chymase 1 (CMA1) protein levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, or serum. Its sensitivity is 0.042 ng/mL, which can accurately detect low concentrations of CMA1 in the sample.
Target
CMA1
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated mast cell Chymase antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell culture supernates, serum
Sample Volume
50 μL-100 μL
Sensitivity
0.042 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.16 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Mast cell chymase 1
Alternate Names
Chymase 1; Chymase 1 mast cell; Chymase 1 preproprotein transcript I; CMA1; EC 3.4.21.39; Mast cell chymase 1; Mast cell protease 3; Mast cell protease 5; MCP3P; Mcpt5; MCT1; MMCP-5
Uniprot No.
P23946
N-Glycosylation Site
80, 103
Application Notes
Application
The quantitative human CMA1 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of CMA1, helping to understand its role in cardiovascular.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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