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Quantitative Human CXCR5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX371

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Specifications
Product Description
The quantitative human CXCR5 (glycosylated) sandwich ELISA kit is designed to detect human chemokine C-X-C-motif receptor 5 (CXCR5) levels. CXCR5 is a G protein-coupled receptor, which binds to CXCL13. CXCR5 plays a role in B cell migration. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.096 ng/mL, which can accurately detect low concentrations of CXCR5 in the sample.
Target
CXCR5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.096 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Chemokine C-X-C-motif receptor 5
Alternate Names
CXCR5; Chemokine C-X-C-motif receptor 5; CD185; BLR1; MDR15; Monocyte-derived receptor 15
Uniprot No.
N-Glycosylation Site
28, 196
Application Notes
Application
The quantitative human CXCR5 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CXCR5 levels. It is useful for studying the role of CXCR5 in B cell homing to lymph nodes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-110%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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