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Quantitative Human LAMβ3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX575

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Specifications
Product Description
The quantitative human LAMβ3 (glycosylated) sandwich ELISA kit is designed to detect human laminin β3 (LAMβ3) levels. LAMβ3 is a protein that is a component of laminin. Laminins are a family of extracellular matrix proteins. They are major components of basement membranes. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.253 ng/mL, which can accurately detect low concentrations of LAMβ3 in the sample.
Target
LAMβ3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
0.253 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-50 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Laminin β3
Alternate Names
LAMβ3; Laminin β3; LAM-B3; Laminin-5 subunit beta
Uniprot No.
N-Glycosylation Site
220, 604, 810
Application Notes
Application
The quantitative human LAMβ3 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and matrix biology, particularly in studies of basement membranes. Researchers can measure LAMβ3 levels in tissues. Applications include studies of tissue development and skin diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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