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Quantitative Human NEFL (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX187

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Specifications
Product Description
The quantitative human NEFL (glycosylated) sandwich ELISA kit is designed to detect human neurofilament (NEFL) levels. NEFL is a major component of neuronal cytoskeletons, providing structural support to axons and playing a crucial role in axonal transport. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 6.885 pg/mL, which can accurately detect low concentrations of NEFL in the sample.
Target
NEFL
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
6.885 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
25 pg/mL-800 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Neurofilament
Alternate Names
NEFL; Neurofilament; CMT1F; NF68; NFL; Neurofilament triplet L protein
Uniprot No.
O-Glycosylation Site
21, 27
Application Notes
Application
The quantitative human NEFL (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for diagnosing and monitoring neurodegenerative diseases, traumatic brain injury, and other neurological disorders. Researchers and clinicians utilize this ELISA to quantify NEFL levels, providing insights into axonal injury and disease progression.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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