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Quantitative Human NEFL (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX187)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NEFL (glycosylated) sandwich ELISA kit is designed to detect human neurofilament (NEFL) levels. NEFL is a major component of neuronal cytoskeletons, providing structural support to axons and playing a crucial role in axonal transport. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 6.885 pg/mL, which can accurately detect low concentrations of NEFL in the sample.
Target NEFL
O-Glycosylation Site 21, 27
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 6.885 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 25 pg/mL-800 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Neurofilament
Alternate Names NEFL; Neurofilament; CMT1F; NF68; NFL; Neurofilament triplet L protein
Uniprot No. P07196
Application The quantitative human NEFL (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for diagnosing and monitoring neurodegenerative diseases, traumatic brain injury, and other neurological disorders. Researchers and clinicians utilize this ELISA to quantify NEFL levels, providing insights into axonal injury and disease progression.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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