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Quantitative Rat NT5E/CD73 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX820

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Specifications
Product Description
The quantitative rat NT5E/CD73 (glycosylated) sandwich ELISA kit is designed to detect rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 0.168 mIU/mL, which can accurately detect low concentrations of NT5E/CD73 in the sample.
Target
NT5E/CD73
Sample Types
Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.168 mIU/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.65 mIU/mL-40 mIU/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Rat
Full Name
5'-Nucleotidase/cluster of differentiation 73
Alternate Names
5'-Nucleotidase/cluster of differentiation 73; Nte5'-nucleotidase; 5'-NT; EC 3.1.3.5; Ecto-5'-nucleotidase; CD73 antigen; CD73
Uniprot No.
N-Glycosylation Site
55, 313, 335, 349, 405
Application Notes
Application
The quantitative rat NT5E/CD73 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of NT5E/CD73, helping to understand its role in cardiovascular.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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