Explore Glycoprotein Interactions and Immune Responses with Creative Biolabs' Specialized ELISA Solutions.
Glycoproteins are complex molecules formed by the combination of proteins and sugar chains, widely present in cell membranes, serum, and various body fluids. Glycoproteins play a key role in different biological processes, especially in the immune system, where they can act as antigens to trigger immune responses. Therefore, the development of glycoprotein-based immunology assay kits is critical. Creative Biolabs has a strong scientific research team, and we have developed and launched a series of high-quality glycoprotein-based immunology assay kits that meet market needs, such as the glycoprotein screen ELISA kit and anti-glycoprotein antibody ELISA kit. These ELISA kits are essential tools for detecting glycoproteins in various sample types and are widely applied in biochemical and medical research. Moreover, they also provide important support for basic research and drug development and promote progress in glycoprotein-related fields.
The detection principle of ELISA kits is to use enzyme-labeled antibodies or antigens as probes, through the specific binding of antigens and antibodies, and then through a series of reactions catalyzed by enzymes to display measurable signals. The main steps are as follows:
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Coating: Fix specific antigens or antibodies on microplates.
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Add sample: Add the sample to be tested (such as serum, plasma, cell culture supernatant, etc.) to the microplate, and the antibody or antigen in the sample reacts with the antigen or antibody bound to the plate. In this step, the antigen and antibody form a complex.
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Washing: Remove unbound components by washing them to reduce interference.
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Secondary antibody incubation: Add enzyme-labeled secondary antibody for incubation.
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Substrate reaction: Add substrate solution, and the enzyme catalyzes the substrate reaction to produce a detectable colored product. Tetramethylbenzidine (TMB) is usually used as a substrate.
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Measurement: After the enzyme reaction is completed, the signal intensity of the colored product is measured at a specific wavelength and calculated according to the standard curve. The signal is usually proportional to the amount of antigen or antibody in the sample.
Glycoprotein-based immunology assay kits provided by Creative Biolabs are a type of kit used to detect and analyze the presence and level of glycoproteins in biological samples. They are used for different types of complex biological samples, including serum, plasma, cell extracts, etc. These kits are capable of detecting glycoproteins down to the picomolar level and also ensure rapid detection and quantification of specific glycoproteins through the selection of specific antibodies. Please feel free to contact us if you would like to consult more information about our glycoprotein-based immunology assay kit products.
