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GlycoErase™ ALG5 Knockout Human Malignant Melanoma Cell A375

CAT#: GLJF-0525-JF1156

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Overview Specification Target Information Related Products
Overview
Description
The ALG5 deficient A375 cell line serves as an important model for analyzing early glucosylation events within the N-glycosylation pathway, as the absence of ALG5 perturbs both N-glycan biosynthesis and protein folding processes, enabling researchers to dissect the impact of N-glycosylation defects on melanoma cell survival, metastatic potential, interactions with the surrounding microenvironment, and to facilitate the screening of agents that modulate N-glycosylation.
Product Type
KO Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:3~1:4
Cell Line
A375
Primary Disease
Melanoma
Lineage
Skin
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
DMEM & FBS & Glutamine
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
ALG5
Full Name
ALG5 dolichyl-phosphate Beta-glucosyltransferase
Alternative Name
bA421P11.2
Location
13q13.3
Gene ID
29880
Summary
Essential for oligomannose core glucosylation (N-linked); ensures substrate recognition, enabling efficient transfer to glycoproteins - critical for proper protein folding and function.
For Research Use Only.
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