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GlycoErase™ HEK293T ALG9 Knockout Cell Line

CAT#: GLJF-0425-JF390

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Overview Specification Target Information Related Products
Overview
Description
Researchers studying lipid-linked oligosaccharide assembly and its role in N-linked glycosylation will find the ALG9 knockout HEK293T cell line to be a valuable asset. This cell line, lacking the ALG9-encoded alpha-1,2-mannosyltransferase, allows for investigations into the functional consequences of disrupting this crucial step in N-glycan biosynthesis.
Product Type
KO Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:2~1:4
Cell Line
HEK293T
Lineage
Embryonic kidney
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
DMEM & FBS & Penicillin/Streptomycin
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
ALG9
Full Name
ALG9 alpha-1,2-mannosyltransferase
Alternative Name
DIBD1
Location
11q23.1
Gene ID
79796
Summary
Facilitates lipid-linked oligosaccharide assembly; mutations lead to CDG II (congenital disorder of glycosylation type II); multiple transcript variants (different isoforms) may influence enzyme specificity.
For Research Use Only.
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