GlycoErase™ SIGLEC5 Knockout A549 Cell Line (CAT#: GLJF-0825-JF886)

Description	GlycoErase™ SIGLEC5 knockout A549 cell line is a human lung epithelial cancer model lacking SIGLEC5 expression. SIGLEC5 belongs to the CD33-related Siglec subset and functions as a sialic acid-binding lectin with Ig-like structural domains. It is known to suppress the activation of monocytes, macrophages, and neutrophils, and also plays a role in immune evasion by group B Streptococcus (GBS). The knockout of SIGLEC5 in the A549 background offers a versatile system to investigate innate immune regulation, host-pathogen interactions, and bacterial immune escape mechanisms, while also serving as a platform for therapeutic development and functional immunology studies.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:3~1:6
Cell Line	A549
Primary Disease	Non-small cell lung cancer
Lineage	Lung
Lineage Subtype	Non-small cell lung cancer

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	F-12K & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC5
Full Name	Sialic Acid Binding Ig Like Lectin 5
Alternative Name	CD170, OBBP2, CD33L2, OB-BP2, SIGLEC-5, Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	8778
Summary	This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.