GlycoErase™ SIGLEC5 Knockout A549 Cell Line

GlycoErase™ SIGLEC5 knockout A549 cell line is a human lung epithelial cancer model lacking SIGLEC5 expression. SIGLEC5 belongs to the CD33-related Siglec subset and functions as a sialic acid-binding lectin with Ig-like structural domains. It is known to suppress the activation of monocytes, macrophages, and neutrophils, and also plays a role in immune evasion by group B Streptococcus (GBS). The knockout of SIGLEC5 in the A549 background offers a versatile system to investigate innate immune regulation, host-pathogen interactions, and bacterial immune escape mechanisms, while also serving as a platform for therapeutic development and functional immunology studies.

KO Cell Lines

Human

Epithelial-like, adherent

1:3~1:6

A549

Non-small cell lung cancer

Lung

Non-small cell lung cancer

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

F-12K & FBS & Penicillin/Streptomycin

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC5

Sialic Acid Binding Ig Like Lectin 5

CD170, OBBP2, CD33L2, OB-BP2, SIGLEC-5, Sialic Acid Binding Ig Like Lectin

19q13.41

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.