GlycoErase™ SIGLEC5 Knockout Jurkat Cell Line (CAT#: GLJF-0825-JF891)

Description	GlycoErase™ SIGLEC5 knockout Jurkat cell line is a Jurkat model in which SIGLEC5 expression has been deleted. SIGLEC5 belongs to the CD33-related subset and suppresses activation of monocytes, macrophages, and neutrophils, and also mediates bacterial immune evasion. The knockout Jurkat line offers a platform to investigate innate immune regulation, host-pathogen interactions, and inhibitory signaling, supporting applications in T cell immunology, cytokine profiling, and immunotherapy development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC5
Full Name	Sialic Acid Binding Ig Like Lectin 5
Alternative Name	CD170, OBBP2, CD33L2, OB-BP2, SIGLEC-5, Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	8778
Summary	The gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins feature structural motifs in their immunoglobulin-like domains, along with sialic acid recognition sites in the first Ig V-set domain. The encoded protein belongs to the CD33-related subgroup of Siglecs and inhibits the activation of monocytes, macrophages, and neutrophils. It also contributes to immune evasion by Group B Streptococcus (GBS).