GlycoErase™ SIGLEC8 Knockout A549 Cell Line (CAT#: GLJF-0825-JF904)

Description	GlycoErase™ SIGLEC8 knockout A549 cell line is a human lung epithelial cancer model lacking SIGLEC8 expression. SIGLEC8 belongs to the CD33-related Siglec subgroup and contains conserved intracellular motifs, including an immunoreceptor tyrosine-based inhibitory motif (ITIM) and a SLAM-associated protein (SAP) binding motif, which mediate inhibitory signaling in immune cells. Removal of SIGLEC8 in the A549 background provides a specialized system to explore immune checkpoint regulation, inhibitory signaling pathways, and hematopoietic cell interactions, while enabling applications in immunology research, disease mechanism studies, and therapeutic development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:3~1:6
Cell Line	A549
Primary Disease	Non-small cell lung cancer
Lineage	Lung
Lineage Subtype	Non-small cell lung cancer

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	F-12K & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC8
Full Name	Sialic Acid Binding Ig Like Lectin 8
Alternative Name	SAF2, SIGLEC-8, SIGLEC8L, Sialic Acid Binding Ig Like Lectin
Location	19q13.33-q13.41
Gene ID	27181
Summary	Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490).