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| Description | GlycoFlux™ MSCV-ALG9 Viral Particle harnesses MSCV to express ALG9 for lipid-linked oligosaccharide extension; the genome-integrating, replication-defective format supports durable expression in stem/progenitor systems and comparative analyses of isoform-specific substrate preferences linked to CDG II. |
| Product Type | Recombinant MSCV Retrovirus |
| Target | ALG9 |
| Species | Human |
| Promotor | MSCV 5' LTR promoter |
| Packaging System | Retrovirus |
| Packaging Cell | HEK293T |
| Application | This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome. |
| Shipping | Dispatched on dry ice with temperature held at or below -80°C. |
| Storage | Store at -80°C to preserve titer and stability. |
| Handling Notes | Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection). |
| Product Disclaimer | These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application. |
| Titer Assay | qPCR |
| Mycoplasma Test | Negative |
| Sterility | Sterility testing confirmed no microbial contamination. |
| Transduction Evaluation | Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output. |
| Insert Identity Confirmation | All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration. |
| Full Name | ALG9 alpha-1,2-mannosyltransferase |
| Alternative Name | DIBD1 |
| Location | 11q23.1 |
| Gene ID | 79796 |
| Summary | Involved in lipid-linked oligosaccharide assembly; mutations cause CDG II; isoforms may differ in substrate preference. |