GlycoSens™ 6-Phosphofructokinase (PFK) Assay Kit, Micro-method

The GlycoSens™ 6-Phosphofructokinase (PFK) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of 6-phosphofructokinase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Glycolysis Series

PFK

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

5 μL - 20 μL

Target 6-phosphofructokinase (PFK) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target PFK.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

6-Phosphofructokinase

6-Phosphofructokinase; PFK

Functioning at the core of various biological processes, 6-phosphofructokinase (PFK) is a key subject of biochemical analysis. This enzyme operates as a critical regulatory switch, modulating the activity of downstream signaling proteins. In addition, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Optimized for high-throughput profiling of 6-phosphofructokinase (PFK) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.